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Sterilization is the process of killing all forms of microbial life in or on the given object or preparation. Microbiologically, sterile material is one that contains no living organisms at all and the term sterile is therefore an absolute one. Accordingly, an object is sterile or non sterile but it can never be semisterile or almost sterile. 
DEFINITIONS OF TERMS RELATED TO STERILIZATION:  The exponential relationship between number of organisms living and the extent of treatment is studied from the inactivation kinetics of pure culture of organisms by exposing them to physical or chemical sterilization procedures. Survivor curves have been used to develop inactivation data for a specific sterilization procedure using suitable biological indicator. This data is helpful in establishing a sterilizing regimen for a specific preparation. 
D value: It is the parameter calculated as the time taken for one log (90%) reduction in the number of microorganisms. F value: This value is used to compare the lethality of different heat sterilization procedures....